Limited reverse transcriptase activity of phi29 DNA polymerase
نویسندگان
چکیده
منابع مشابه
Evolving thermostable reverse transcriptase activity in a DNA polymerase scaffold.
DNA polymerases are involved in all DNA synthesis that occurs in nature. Furthermore, DNA polymerases are the workhorses in numerous important molecular biological core technologies like the widely applied polymerase chain reaction (PCR), cDNA cloning, genome sequencing, and diagnostics based on nucleic acids. In each of these processes the DNA polymerase requires a DNA strand as a template for...
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The kinetic pathway of DNA-dependent DNA polymerase activity of human immunodeficiency virus reverse transcriptase (HIV RT) as determined by pre-steady-state methods using a defined primer/template is as follows, [formula: see text] where E is RT, Dn,n+1 is primer/template, dNTP is deoxyribonucleoside triphosphate, and PPi is pyrophosphate. The rate-determining step for enzyme turnover in singl...
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Reverse transcriptase polymerase chain reaction (RT-PCR) has become a well-established and powerful molecular technique for studying ribonucleic acids. It is used in medical diagnostics for detecting viral RNA, in hematology and oncology for detecting chimeric transcripts of rearranged genes (1), and in the broad area of research applications in gene expression studies. Since its introduction (...
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Polymerases are a family of enzymes responsible for copying or replication of nucleic acids (DNA or RNA) templates and hence sustenance of life processes. In this paper, we present a method to exploit a strand-displacing polymerase phi29 as a driving force for nanoscale transportation devices. The principal idea behind the device is strong strand displacement ability of phi29, which can displac...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2018
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gky190